By Robert F. Whitcomb
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Extra resources for The Mycoplasmas. Spiroplasmas, Acholeplasmas, and Mycoplasmas of Plants and Arthropods
Oculi. At least one strain in this collection (0502) could not be identified as a recognized Acholeplasma species, although the organism grew well in the absence of serum. This organism has also been the subject of further study (unpublished data from our laboratory and that of J. M. Bove). The 0502 strain appears to be serologically unrelated to established species in the class Mollicutes and does not share serologic properties with other plant acholeplasmas (A. florum or J233). Optimum growth occurred at 30°C, with a temperature range of 23°-37°C.
In addition, filtration of early broth cultures through membrane filters of 220-nm pore diameter can exclude the likelihood that L-phase variants are present. IV. CONCLUSION AND FUTURE OUTLOOK The evolution of the systematics of wall-less prokaryotes, based on the framework of features delineated for the class Mollicutes, has provided what is currently perceived by most mollicutologists as a reasonable and logical approach to classifying and identifying a widely diverse group of microorganisms.
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