By Harris Busch (Eds.)
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However, biologists have used this property of somatic-cell hybrids to locate certain human genes on human chromosomes. The best example is the gene determining thymidine kinase of man. y 1963). These altered cells will not grow in medium containing hypoxanthine, aminopterin, and thymidine ( HAT ) ( Littlefield, 1964). When such murine cells fuse with a human cell, the cell hybrids will be able to perpetuate because of the thymidine 1. Mammalian Chromosomes 25 kinase gene in the human genome. The loss of the human chromosome carrying the kinase gene will cause lethality of the hybrid.
Sei. US 68, 2699. Miller, Ο. J . , Allderdice, P. , Miller, D. , Breg, W. , and Migeon, B. R. (1971a). Science 173, 244. Miller, O. J . , Miller, D. , Kouri, R. , Allderdice, P. , Dev, V. , Grewal, M. , and Hutton, J. J . ( 1971b). Proc. Nat. Acad. Sei. S. 68, 1530. Mittwoch, U. ( 1967 ). " Academic Press, New York. , Grumbach, M. , and Taylor, J . H. ( 1 9 6 2 ) . Proc. Nat. Acad. Sei. S. 48, 756. 32 FRANCES Ε . , and Bodmer, W. (1970). Nature (London) 223, 358. Ohno, S. ( 1 9 6 7 ) . " Springer-Verlag, Berlin and New York.
And Shaw, M. W. ( 1 9 7 1 ) . Proc. Nat. Acad. Set. S. 68, 2073. Duffey, P. A. ( 1 9 7 2 ) . Science 176, 1333. , and Lejeune, J . (1971). C. R. Acad. Sei. Paris 272, 2638. , de Grouchy, J . , Finaz, C , and Lejeune, J . ( 1 9 7 1 ) . C. R. Acad. Sei. Paris 273, 587. Egozcue, J . , Perkins, Ε. , and Hagemenas, F. ( 1 9 6 8 ) . Folia Primat. 9, 81. Fredga, K. ( 1965). Hereditas 52, 411. Fredga, K. (1967). Hereditas 57, 421. Fredga, K. ( 1970). Phil. Trans. Roy. Soc. London Β 259, 15. ( 1972). Exp.