By Harris Busch (Eds.)

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3 . CONDENSED CHROMATIN As a counterpart to the above opinions, there are two investigations supporting the thesis that not only the dispersed chromatin and/or the perichromatin region, but also the condensed chromatin itself could be sites of transcription. In Trillium microspores in d phase, after incuba3 tion with [ H]UdR for 60 min followed by various periods of chase, Kemp (1966) observed a shift of activity from the dispersed toward the condensed chromatin. He interpreted these results as supporting the occurrence of chromatin dispersion during transcription, since the spores in d were not replicating DNA.

Marinozzi (1963) succeeded in staining preferentially histone-containing material by silver impregnation on acrolein fixed material. The use of ammoniacal silver followed by formalin reduction was considered by MacRae and Meetz (1970) to distinguish between lysine-rich and arginine-rich histones at the ultrastructural level. They followed accumulation of newly synthesized arginine-rich histones throughout the differentiation of the erythroid cell line. Silver was also employed as silver nitrate by Smith and Stuart ( 1 9 7 1 ) , who claimed to stain nucleic acid-associated proteins after perchloric acid extraction of DNA.

They used very short pulses (2 min) and showed transcription to occur first in the perichromatin region. When short pulses were followed by chases, a shift of activity from this region to the interchromatin region was observed. At 15 min, the labeling was found almost evenly throughout the nucleus, and even more so at 60 min. They related the perichromatin activity to the rapidly labeled HnRNA, and therefore to the messenger RNA. These data point to one of the possible interpretations of Milner and Hayhoe ( 1 9 6 8 ) , by strongly suggesting that the activity is first detected in the perichromatin region, and secondarily migrates into the euchromatin, as opposed to the alternative hypothesis, which suggests that both the perichromatin 1 .

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