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Additional resources for Proteins and Nucleic Acids. A Comprehensive Treatise
In both cases, less than 1% mismatch was detected in duplexes long enough to be excluded from the agarose column. Data for pea (Thompson, 1976a) and soybean (Goldberg, 1978) DNA are quite similar. The precise significance of these observations remains to be determined (see Section I,G). In the case of higher plants, however, one quite trivial hypothesis must often be considered when evaluating data obtained with total leaf DNA preparations, since chloroplast DNA sequences would be expected to form long, precisely paired duplexes.
Many transposons are characterized by inverted repeats at their termini, and similar structures are known to be widely scattered throughout eukaryotic genomes (see Section I,E,5). , 1976), or that the long regions are tandem repeats which maintain their internal homogeneity by "crossover fixation" (Smith, 1976). If the presumed precursor-product relationship holds, one might ex pect to find some sequence homology between the long and short repetitive fractions. In accord with this prediction, Wu et al.
As defined by Davidson et al. (1975a), the Xenopus pattern is characterized by single-copy lengths of about 800 to several thousand nucleotides with a large fraction less than 1500 nucleotides in length, while those m Drosophila often exceed 10,000 nucleotides without interruption. However, to group all higher-plant genomes together in the Xenopus pattern would be an unfortunate oversimplification. , 1978a. 34 William F. Thompson and Michael G. 6 1800 >3600 1200 >9000 1000 >5000 1400 >4300 25 <40 40 <20 20 <11 26 <11 20 10 300 1000 >2300 1000 >9000 300 1500 3500 >6000 Reference6 < 6 15 <10 13 7 5 < 5 a Interpretations of similar data may differ in reports from different laboratories.