By Michael J. Soares, Joan S. Hunt
A suite of state-of-the-art laboratory ideas for the examine of the trophoblast and placental biology. The ideas offered variety from experimental animal types, to animal and human placental organ and cellphone tradition structures, to morphological, biochemical, and molecular techniques for assessing trophoblast/placental progress, differentiation and serve as. quantity 2 offers effortlessly reproducible protocols for learning trophoblast invasion, maternal immune mobile responses to being pregnant, and the hormone generating features of the placenta. There also are easy methods to examine the barrier functionality of the placenta, its position in regulating the delivery of food and wastes, and the variations of trophoblast cells and the placenta to illness. Highlights contain recommendations to investigate trophoblast telephone interactions with the uterine vasculature and immune cells, and the endocrine services of the placenta. A significant other first quantity concentrates on tools for examine embryo-uterine implantation, trophoblast mobilephone improvement, and the association and molecular characterization of the placenta.
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Extra info for Placenta And Trophoblast: Methods And Protocols
1. 1. Collection of Vaginal Smears 1. Grasp the tail of a mouse with the thumb and forefinger of one hand. 2. Place the mouse on the top of the cage cover (wire top). As the mouse attempts to move forward, quickly grasp the loose skin at the back of the neck using the thumb and forefinger of the other hand. The head of the mouse will be immobilized, if the skin is held properly. 3. Lift the mouse in your hand and secure the tail between the small finger and the palm of the same hand. 4. Keep the face of the mouse up and locate the vagina.
No. 01-804). 11. 9% sodium chloride, Baxter Healthcare Corporation, cat. no. 281324). 2. INDUCTION OF PSEUDOPREGNANCY 1. Vasectomized male mice. 2. Sexually mature female mice (45–50 d old). 3. , cat. no. 11152-10). 3. HORMONAL PRIMING OF OVARIECTOMIZED MICE 1. Ovariectomized females rested for 10–15 d. 2. , cat. no. S-3547). 3. , cat. no. 309623). 4. , cat. no. P-1030). 5. , cat. no. E-8875). 6. Hotplate (Fisher Scientific, cat. no. 11-497-6A). 4. INDUCTION OF DECIDUALIZATION BY ARTIFICIAL MEANS 1.
Huet-Hudson, Y. , and Dey, S. K. (1993) Blastocyst’s state of activity determines the “window” of implantation in the mouse receptive uterus. Proc. Natl. Acad. Sci. USA 90, 10,159–10,162. 9. Paria, B. , Lubahn, D. , Dey, S. , and Das, S. K. (1999) Uterine decidual response occurs in estrogen receptor-α-deficient mice. Endocrinology 140, 2704–2710. 10. Wordinger, R. , Jackson, F. , and Morrill, A. (1986) Implantation, deciduoma formation and live births in mast cell-deficient mice (W/Wv). J. Reprod.