By Z. à esták, J. Čatský (auth.), Zdenĕk à estăk (eds.)

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With increasing leaf age the numbers of chloroplasts per palisade parenchyma cell and spongy parenchyma cell increase substantially (Figs. 3). The number of plastids in palisade parenchyma cells increases until the leaf growth ceases, 20 ... 30 90 E ... :I. II: II: w III 1: :J ... 70 • PALISADE w Iw 1: 7 Z ... « 1-111 Q I/IU «II: 50 "'w ~II. • II: 9 1: • U •• I1/1 « • ... II. SPONGY 0 II: 0 Phaseo/us vulgaris ... « c 120 . II. o II: 0 ... 80 w 1: Sinapis alba U II: . w II. Triticum oestivum 1001/1 Q j: 1/1 « 40 ...

These studies are very prospective for physiological leaf anatomical investigations. 3 Intercellular Air Spaces and Internal Leaf Surface Through intercellular air spaces, CO2 is transported in the gaseous phase from the stomata to the mesophyll cell surface. The internal leaf surface is usually 10 to 40 times larger than the external leaf area depending on the plant species, environmental conditions, etc. The parts of mesophyll cells contributing to the internal leaf surface are more or less covered with chloroplasts (80% in palisade parenchyma and 20% in spongy parenchyma cells, cf.

21 Phaseolus vulgaris L. primary leaf, 1 to 14 dafter cv. Canadian Wonder germination (pots, growth chamber) leaf 5, 3 to 65 d (leaf area 4 to 605 cm 2) leaves 8, 12, 16, 20 H elianthus annuus L. (field) Meier and Lichtenthaler 1981 Whatley 1980 Nekrasova 1978 Mokronosov 1981 Bourdu et al. 1975 Shul'gin et al. 1977 >f:o.. 0:. •540 number [ceU-l]: PP: 40... 215 ... •85•.. 350... 280 SP: 80... 65 leaf 13: PP: 60... 140... •60•. 40 number per mm 2 [x 103 ]: leaf 5: 580•.. 44.. 13 ... 66 ••. 50 Age of l~af or leaf insertion Chloroplast parameter (from young to Qeaves numbered from the old or from upper to lower leaves) oldest one) (2) (3) Spinacea oleracea leaves 4 and 5, 10 to 24 d L.

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