By Pauline Alderson, Martin Rowland

Revised in attention of alterations made within the ULEAC syllabus B for Biology and the improvement of the nationwide Curriculum for technology, this publication emphasizes the functions of Biology. it's been up-to-date all through with sections improved and further to supply updated info.

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Genetic engineering will in future give us many enzymes at a reasonable cost. For the time being we shall continue to collect those produced naturally, such as rennin, and others produced by bacteria and fungi. ( a) Economic advantages of enzymes When we get enzymes to work for us in factories , costly and complicated chemical processes can be carried out more efficiently and more cheaply. There is no need for high temperatures and pressures. Several enzymes, especially tho se secreted in large amounts during the digestion processes of bacteria and fungi, have been collected and used for many years.

Even though the pipette will already have been sterilised, you should flame it along its whole length and cool it before putting it in the culture. You should not flame it after using it because this would spray the culture's microorganisms into the air. ( vi) Aseptic disposal of contaminated material All equipment and media must be sterilised before being stored or thrown away. Pipettes must be put in a tall container filled with disinfectant. Wire loops must be flamed. Containers such as tubes, bottles and Petri dishes must be autoclaved (even though plastic Petri dishes will melt and will have to be thrown away).

Use the spirit marker and a ruler to draw two lines at right angles to each other across the middle of the base of the dish . Write Rhizopus (the name of the fungus to be grown), your initials and the date near the edge of the dish. Turn the dish the right way up and leave it on the bench. 3. Take a test-tube of molten agar from the water bath. 11). Immediately pour the contents of the test-tube into your Petri dish, lifting the lid at an angie so th at only the very top of the test-tube gets over the dish.

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