By Carroll N. Smith

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It is different, for humoral and immunological reasons, if the host has had pre­ vious experience of the parasite, to such an extent that for many species the same individual host can hardly be used more than once (see Chapter 4 by Gregson). The mites themselves will be subject to fluctuating physiological states and therefore varying requirements and behavior patterns, and they will usually attach on their own initiative to the host at the optimum time. For example, mites which normally feed at night will continue to do so in the laboratory.

Hosts: mice; adults fed singly on 6- to 8-day-old suckling mouse in cotton-stoppered vial. This important species has not yet been satisfactorily colonized. 2. Macronyssidae Ornithonyssus bacoti (Hirst, 1913), Tropical rat mite: Bertram, Unsworth, and Gordon (1946), Cross (1954), Ohmori (1935, as Liponyssus nagayoi Yamata, synonym), Skaliy and Hayes (1949). Life cycle: egg 1-4 (unfertilized eggs develop into fertile males); larva 1 (no feed); protonymph 5-14 (requires at least one feed); deuto­ nymph 1-2 (no feed).

INTRODUCTION 9 organism. The most advanced insect pathogen for practical control, currently, is "Bacillus thuringiensis. This organism can be produced on artificial media. However, there is no assurance that artificial media will provide the means for the production of most of the prom­ ising insect pathogens now known that could conceivably be used for effective insect control. The milky spore disease of the Japanese beetle, Popillia japonica Newman, may serve as an example of the difficulties that may be involved in the mass production of the path­ ogen on noninsect media.

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