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187 (1977). Immun, and Immunopath. 551 (1977). , Immunol, and Immunopath. 8, 8, Clin. 1 (1977). S. A. H. ), p. 75. Academic Press, New York, (1976). 12. , IDEM 1976, 387 (1976). 13. , and 11. , Clin. 14. Immun, and Immunopath. 10, (1978). , J. Meth. 17, 319 (1977). 214 Immunol. 39 SECTION I ANIMAL MODELS 15. , 16. , Int. Int. Arch. Arch. Allergy Allergy Appl. Appl. Immunol. Immunol. 53, 49, 152 (1977). 670 (1975). 40 IMMUNE REGULATORS IN TRANSFER FACTOR DISCUSSION FUDENBERG: I noticed I think on slide 8 you had your SK-SD positive and the last one was negative by skin test; yet that individual gave 3,000 CPM T s in the presence of antigen, versus 74 CPM's I believe in the absence of antigen.

KLESIUS: In the paper that I will present next, we thoroughly tested out with this particular PPD preparation any mitogen like activity, and we have not found any. In the protein technique, the highest amount per half a million cells that we use is 5/10 of a ug/culture, which is appreciably lower than you would use in the thymidine technique. SECTION I ANIMAL MODELS ASCHER: You said lysate. 25 This is dialysate, is it not? KLESIUS: Right. Dialysate. ASCHER: A word about the alcohol. We have talked about this before.

Cells were purified in order to increase lymphocyte concentration by separation on sucrose gradient (d 1,18 - 1,20), centrifugation and red cell lysis by ammonium chloride (6). These preparations always contained more than 65 % of viable lymphocytes. The HDLE batches from high or low responders were prepared as previously described (1-5): cell pellets were centrifuged and the supernatants were dialyzed overnight using 20 DC Visking dialysis tubing against distilled water. The dialysates were lyophilized.

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