By Professor Dr. Y. P. S. Bajaj (eds.)

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All plantlets regenerated from embryos were diploid, however. 3 Embryo Rescue Consumers of table and raisin grapes prefer seedless to seeded cultivars. The breeding of stenospermocarpic seedless varieties is hampered by a number of problems (Cain et al. 1983), mentioned above. Embryo abortion at an early age seems to be involved in stenospermocarpy (Einset and Pratt 1975). Cain et al. (1983) have developed an in vitro technique for the culture of normally abortive grape ovules and for their subsequent germination.

J Am Soc Hortic Sci 102:360-363 Lakso AN, Reisch BI, Mortensen J, Roberts MH (1986) Carbon dioxide enrichment for stimulation of growth of in vitro-propagated grapevines after transfer from culture. J Am Soc Hortic Sci 111 :634-638 Lebrun L (1985) Isolement et culture de protoplastes de vigne. In: Proc Moet-Hennessy ColI Amelioration de la vigne et culture in vitro, Paris, p 215 Lebrun L, Rajasekaran K, Mullins MG (1985) Selection in vitro for NaCI-tolerance in Vitis rupestris Scheele. Ann Bot (London) 56:733 - 739 Lee TC, Wicks T (1982) Dual culture of Plasmopara viticola and grapevine and its application to systemic fungicide evaluation.

In: Evans DA, Sharp WA, Ammirato PV and Yamada Y (eds) Handbook of plant cell culture, volt. Macmillan, New York, pp 228-287 Bajaj YPS (1986) In vitro preservation of genetic resources - Thchniques and problems. In: Proc Int Symp Nuclear techniques and in vitro culture for plant improvement. ) from fragmented shoot apices. Vitis 17:335 - 340 Barlass M, Skene KGM (1980a) Studies on the fragmented shoot apex of grapevine. I. The regenerative capacity of leaf primordial fragments in vitro. J Exp Bot 31 :483 - 488 Barlass M, Skene KGM (1980b) Studies on the fragmented shoot apex of grapevine.

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