By P. Vijay Sarthy (auth.), S. Robert Hilfer, Joel B. Sheffield (eds.)

The eye has interested scientists from the earliest days of organic in­ vestigation. the range of its elements and the precision in their interplay make it a favourite version procedure for quite a few developmental stories. the attention is a very beneficial experimental method not just simply because its tissues supply examples of primary procedures, but additionally since it is a sought after and simply obtainable constitution at very early embryonic a while. which will offer an open discussion board for investigators engaged on all facets of ocular improvement, a sequence of symposia on ocular and visible devel­ opment used to be initiated in 1973. a massive goal of the symposia has been to foster communique among the fundamental examine employee and the medical group. it really is our feeling that a lot might be realized on each side from this interplay. the assumption for a casual assembly permitting greatest ex­ switch of rules originated with Dr. Leon Candeub, who provided the nec­ essary motive force that made the sequence a truth. each one symposium has focused on a unique element of ocular improvement. audio system were chosen to process comparable issues from diverse perspectives.

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Meshul, C. , F. J. Seil, and R. M. Herndon. 1987. Astrocytes playa role in regulation of synaptic density. Brain Res. 402:139-145. Nork, T. , I. H. L. Wallow, S. J. Sramek, and G. L. Anderson. 1987. MUller cell involvement in proliferative diabetic retinopathy. Invest. Ophthalmol. Vis. , Suppl. 28:123. , D. Turner and C. Cepko. 1987. Lineage analysis in the vertebrate nervous system by retrovirus-mediated gene transfer. Proc. Natl. Acad. Sci. USA 84:156-160. Ramon y Cajal, S. 1892. The Structure of the Retina.

At very early stages of development, the 5A11 antibody immunostains much of the embryo. Figure 3 shows that at three days of development the early retina, lens and brain stain well and the head mesenchyme also shows significant labelling. In comparison, CA-II antibodies label the ectodermal portions of the eye and certain scattered cells in the head mesenchyme. Two days later however, the 5A11 staining in tissues outside the eye has decreased dramatically and the presumptive retinal pigment epithelium (RPE) and retina show most intense labelling.

Fluorescently labeled eyebud fragments were grafted into equivalent positions in unlabeled host eyebuds at stage 30-32 (figure 1A). The grafted fragments from opposite poles of the eye were labeled with two different dyes (LFD and LRD). Since the dyes emit different wavelengths of light, the projections of the two populations of cells could be visualized and directly compared in the same host animal. The projection patterns from dorsal and ventral fibers were distinct as early as they were observed in the tectum at stage 4041.

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