By P. Narayanasamy
Biological illness administration strategies have emerged as power replacement to chemical program for holding crop illnesses. Biotic and abiotic organic keep an eye on brokers (BCAs) were established to be potent opposed to ailments because of microbial plant pathogens. mixture of biotic and abiotic brokers ends up in synergism and consequent development within the effectiveness of sickness keep watch over. it truly is necessary to assay the biocontrol strength of all isolates/species of fungal, bacterial and viral biocontrol brokers by means of diverse strategies in vitro and less than greenhouse and box stipulations and to exactly determine and differentiate the simplest isolates from much less potent ones via utilising organic, immunological and nucleic acid-based assays.
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Additional resources for Biological Management of Diseases of Crops: Volume 1: Characteristics of Biological Control Agents
Contiguanum was morphologically very similar to P. periilium. The ITS1 sequences of the nuclear ribosomal DNA of P. contiguanum showed homogeneity of 92 % to P. periilium, 86 % to P. 5 % to P. vanterpoolii. Based on the differences in the nucleotide sequences, P. contiguanum was described as a new taxon. This BCA was found to be mycoparasite on Botrytis cinerea causing gray mold disease of grapevine. Further, P. contiguanum was not pathogenic to grapevine (Paul 2000). Another species P. bifurcatum isolated from wheat field was also found to be a mycoparasite of B.
Indicating the possibility of detection and identification of T. atroviride (Stoppacher et al. 2010). The yeast Torulaspora globosa strain IS 112 isolated from sugarcane rhizosphere was found to suppress the development of sorghum anthracnose disease caused by Colletotrichum sublineolum. This strain was characterized by its yeast killer activity. Suspensions of the sensitive strain Saccharomyces cerevisiae NCY1006 and Torulopsis glabrata ATCC 15126 were prepared after cultivation in yeast broth medium containing methylene blue.
Was found to be an ideal candidate for production of MAbs specific to the genus Trichoderma. This enzyme is extracellular and produced constitutively or semiconstitutively. Chromatographically purified β-1,3glucanase from Trichoderma spp. was employed for production of MAb MF2 by a hybridoma cell line. The MAb MF2 recognized a protein epitope specific to the genus Trichoderma. Enzyme-linked immunosorbent assay (ELISA) showed that MF2 antigen produced by actively growing mycelium of T. koningii was constitutive and occurred in the absence of laminarin, a substrate shown to induce production of β-1,3-glucanase.