By Peter Malcolm Wallis, Brian R. Hammond

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15:26­30.  18:1­26.  A) 148:166­168.  71:89­95.  Med.  15:743­750. 64:165­171.  49:164­171.  44:140­173.  140:222­228.  23:850­855.  549­556.  p 578.  Protozool. 32:383­390.  Gastroenterology 71:57­61.  33:1179­1183.  Microbiol. 45:65­74.  80:49­52.  Parasitologia 4:510­514.  12:851­853.  Science 92:416­417. A.  Cysts formed in vitro were both morphologically and immunologically similar to Giardia cysts formed in vivo and also were viable, as demonstrated by the uptake of fluorogenic dyes.  The inability of Giardia trophozoites to undergo encystation in vitro has raised questions as to whether host­ related factors, such as gut­associated microorganisms or nutritive factors and stimuli from the small intestine, might play a role in this process.

For this purpose, effects of inocula of varying number of trophozoites ranging from 1 × 104 to 1 × 107 on HeLa and Vero cells at different intervals (0, 5, 18, 24, 48, 72 and 96 h) after incubation at 37°C were studied. 5 × 105 cells/mL • Corresponding author.  In all the series, the experiments were terminated at different intervals (0 5, 18, 24, 48, 72 and 96 h) after incubation and the coverslips (2 for each interval in each series for the two cell systems studied), were removed from the tubes and processed for light and electron microscopic studies as described below.

26:700­704.  36:295­307.  74:213­215.  298:319­321. Page 39 Studies on the Prevalence of Giardiasis in Czechoslovakia Michal Giboda Institute of Parasitology, Czechoslovak Academy of Sciences, 370 05 Ceske Budejovice Branišovska 31, Czechoslovakia.  The author suggests that the theory of familial occurrence of giardiasis is valid universally.  The prevalence of giardiasis in Czechoslovakia has been reported from a number of sources (1,2,3,7,8,9,11,12,13,14,15,16) and the data are reported in Table 1.

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