By Oliver H. Lowry
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Extra resources for A Flexible System of Enzymatic Analysis
2-6). Since step 2 by definition is first order, v2 = k2[B], where, according to Eq. (2-21), k2 = Vm2/KB. Ultimately, a steady state will be established, at which time vi = v2. When this occurs, Vi = k2[B]max or [B]max = vjk2 (2-38) where [B]max is the steady-state level of B. Thus for the case of a zero-order reaction followed by a first-order reaction, [B]max will be numerically equal to the velocity of the first reaction divided by the rate constant for the second reaction. 33 μΜ. 69/t1/2 [Eq.
Before use, the pipette should be rinsed with deionized water, and, if possible, with the reagent to be used. Because the constrictions and tips are very small, the orifices may become plugged during use if certain precautions are not taken. Pipettes that have been used for protein solutions should be rinsed two or three times with water immediately after use. Any concentrated solution is likely to clog the pipette if left to dry on the walls around the tip and constriction. If the pipette is to be left overnight the last traces of fluid are removed from the tip by sucking in air after rinsing.
This will also help prevent breakage of pipettes, which if designed for small bore tubes, are of necessity somewhat fragile. To make a clean withdrawal of a sample from a small bore tube, the pipette tube is inserted into the meniscus, rather than into the body of the fluid, and is then moved down the side of the tube to keep pace with the meniscus. Similarly, if a substantial volume of fluid is added into a slender tube, delivery is started at the bottom or at the surface of any fluid already present, and the pipette is drawn up along the side of the tube, keeping the tip always in the meniscus.